Developing a Robust Quantitation Assay for Monoclonal Antibodies and Other Proteins on the Octet® Platform

the Octet platform provides analytical methods for measuring antibody and protein concentrations and binding affinities through various stages of drug discovery and development (table 1). Octet systems circumvent many limitations of ELISA and HPLc assays, enabling informed decisions to be made earlier in both drug and process development – particularly when chromatography and other critical separation steps are needed. Octet systems perform automated sample analysis in 96and 384-well microplate formats. the simple Dip and ReadTM approach enables streamlined workflows and rapid quantitation of 96 samples in as little as 20 minutes, or 384 samples in 70 minutes. In a typical quantitation assay, biosensors coated with capture molecules dip into samples and the concentration of the target Rashi Takkar, Applications Scientist, [email protected]; Sriram Kumaraswamy, Director, Marketing Field Applications, [email protected] 10